猪lipasin基因的克隆及其对肝细胞LPL基因转录的影响

doi:10.11843/j.issn.0366-6964.2016.02.027

Abstract

Abstract:

The study aimed to clone the lipasin gene and investigate its effects on LPL gene transcription in liver cell in pig.The porcine lipasin gene was obtained by using homologous electronic cloning techniques and the eukaryotic expression vector was constructed and transfected into hepatocytes，and further to identify its effect on the transcription level of LPL gene.The results showed that lipasin gene was successfully cloned，the segment size was 615 bp，it was deposited to GenBank with the accession number KF017598.The size of open reading frame (ORF) of lipasin was 594 bp and it encoded 197 amino acids.The weight of lipasin molecular and theoretical isoelectric point were 21.99 ku and 6.79，respectively.The recombinant eukaryotic expression vector PB-EGFP-lipasin was successfully constructed and transfected into hepatocyte，realizing the overexpression of lipasin gene.Compared with the control group，the transcription level of LPL mRNA decreased significantly in PB-EGFP-lipasin transfected hepatocyte.These results suggest that lipasin may affect lipid metabolism by inhibiting the LPL gene transcription in hepatocyte.

CLC Number:

S828
S813.3

LI Man-man，WEN Bing-yan，ZHU He-shui，ZHONG Kai，YANG Guo-yu，WANG Yue-ying. Cloning of Porcine lipasin Gene and Its Effect on LPL Gene Transcription in Liver Cell[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 411-416.

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Cloning of Porcine lipasin Gene and Its Effect on LPL Gene Transcription in Liver Cell

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